Root Canal İrragation solutions and medicine in endodontic treatment is available for to use alternative materials (N acetyl cysteine, boric acid, (chitosan) different concentrations mouse fibroblast cell L929 for to Check the Cytotoxicity and Q. aureus Biofilms for to check antibacterial effectiveness of in vitro aspect evaluation was aimed.
Cell culture test for experiment groups; Chitosan 2048ug/ml- 4ug/ml 10 in different concentration, N Acetyl cysteine (NAC) 50 mg/ml- 0.39 mg/ml between 8 in different concentration, Boric Acid (NA) 64 mg/ml- 0.125 mg/ml between 10 Sodium in different concentration Hypochlorite (NaOCl) 10.5%-5.25 %-2.625% rates 3 different prepared in concentration was created. Antimicrobial test for article concentrations Chitosan 1- 0.002mg/ml, NAC 25- 0.195 mg/ml, Boric acid 32- 0.0625mg/ml aspect was carried out. Prepared microplate At 37ºC 18 hour incubation was released. Study Results group intra- and groups inter- data by comparison analysis was done.
Positive control group the one which… To NaOCl according to all experiment groups more is cytotoxic. Chitosan 128 microgram/ml also first acute toxic the effect of has shown. Q. Aureus on MIC value whereas 0.031 mg/ml is. Antimicrobial dose on the border toxic has been found. N Acetyl Cysteine (NAC) MIC value 1,563 mg/ml while first 24 per hour 25-50 mg/ml in doses toxic It has been found. That is antimicrobial dose on the border toxic It is not has been observed. Boric Acid MIC value 4 While mg/ml This at the rate first 24 per hour cytotoxic not while toxic effect dose and to time connected aspect is increasing. NaOCl all in their concentrations and time in the intervals -best antimicrobial agent found however -most cytotoxic aspect has been observed.
Experiment in groups used NAC and Boric Acid antimicrobial dose borderline cytotoxicity in terms of other from groups more Good has been found.
İrrigation, Biofilm ,Cytotoxic ,İnvitro.
Tahir, A. (2025). Alternative Root Canal İrragation Solutions Which Is Non Cytotoxic and High Antibacterial Effectivenes // İn The Case of in Vitro Study Which Is Held in Laboratory. J Nurs Care Repo; 6(1):1-8. DOI : https://doi.org/10.47485/3065-7636.1034