Brucellosis is one of the most common zoonoses in the world, and no effective methods for Brucella clearance completely until now. Therapy of brucellosis requires deeply understanding of mechanism of Brucella infection and immune responses. We collected human blood samples to analyze the difference of immune responses between latent gram-negative bacterium Brucella and general gram-negative bacterium Escherichia coli. To analyze cytokines release in plasma from patients, a multiplex MAP human cytokine/chemokine immunoassay was used. Human coagulation factor XI expression was detected using ELISA following instructions. In comparison with samples from patients infected with latent gram-negative bacterium Brucella, cytokines secreted by Th2 cells increased in patients infected with general gram-negative bacteria Escherichia coli, and also the chemokines, such as monocyte chemotactic protein 1(MCP-1), macrophage inflammatory protein (MIP-1α, MIP-1β). In the other side, in comparison with samples from patients infected with general gram-negative bacterium Escherichia coli, Interferon inducible protein 10 (IP-10) increased in patients infected with latent gram-negative bacterium Brucella, accompany with increased IFN-γ secretion. Meanwhile, we also found only bacterium Brucella can increased factor XI secretion. Understanding immune response and coagulation function during bacterium Brucella infection will help us to find effective methods for Brucella clearance.
The latent gram-negative bacterium Brucella induced different immune responses to general gram-negative bacterium Escherichia coli