Background and Aims: Chronic Kidney Disease (CKD) is one of the most important complications of diabetes. Many physiological and biochemical changes may cause these complications to appear. The objective of this study was to evaluate the variation oxidative stress for the diagnosis and prediction of renal complication in type 2 diabetic patients.
Material & Methods: Sixty voluntary individuals were divided into two diabetic groups with and without CKD complication on which we assayed some biochemical and oxidative stress parameters. Sensitivity and Specificity of Oxidative stress biomarkers in serum were estimated using receiver operating characteristics (ROC) curve design.
Findings: The results obtained in the present study clearly show that diabetes is confirmed by the hyperglycemia observed in all diabetic patients. The results obtained also show a significant increase (P <0.05) in the concentration of urea, creatinine, uric acid, potassium and serum albumin in diabetic patients with nephropathy compared to diabetics. On the other hand, it was shown that a significant increase (P <0.05) in the concentration of MDA, GSH and total thiol in the serum of diabetic patients compared to diabetics with complications of CKD. ROC assay analysis showed that serum GSH and MDA are predictive markers with high specificity (100%) of nephrotic complications of diabetes.
Conclusion: The present study reveals that diabetes induces metabolic and physiological alterations and an imbalance of oxidant / antioxidant status, which can cause nephrotic complications suggesting the use of GSH, MDA as predictive markers for this complication of the disease.
Keywords: Diabetes, CKD, oxidative stress, ROC.